Evaluating methods and protocols of ferritin-based magnetogenetics

Evaluating methods and protocols of ferritin-based magnetogenetics

Blog Article

Summary: FeRIC (Ferritin iron Redistribution to Ion Channels) is a magnetogenetic technique that uses radiofrequency (RF) alternating magnetic fields to activate the transient AUTOMATIC INTERPRETATION OF HIGH RESOLUTION SAR IMAGES: FIRST RESULTS OF SAR IMAGE SIMULATION FOR SINGLE BUILDINGS receptor potential channels, TRPV1 and TRPV4, coupled to cellular ferritins.In cells expressing ferritin-tagged TRPV, RF stimulation increases the cytosolic Ca2+ levels via a biochemical pathway.The interaction between RF and ferritin increases the free cytosolic iron levels that, in turn, trigger chemical reactions producing reactive oxygen species and oxidized lipids that activate the ferritin-tagged TRPV.In this pathway, it is expected that experimental factors that disturb the ferritin ANÁLISIS DE LA DISCLOSURE DE LOS REQUERIMIENTOS DE PAGOS PÚBLICOS: INFLUENCIA DE LA LEGISLACIÓN Y FUNDAMENTOS DE LA TEORÍA CONTABLE expression, the ferritin iron load, the TRPV functional expression, or the cellular redox state will impact the efficiency of RF in activating ferritin-tagged TRPV.Here, we examined several experimental factors that either enhance or abolish the RF control of ferritin-tagged TRPV.

The findings may help optimize and establish reproducible magnetogenetic protocols.